不同产地枳实、枳壳中柠檬苦素含量比较研究

摘要

目的:建立枳实、枳壳中柠檬苦素的含量测定方法,并测定和比较不同产地枳实、枳壳中柠檬苦素的含量.方法:采用高效液相色谱法( HPLC)测定,色谱柱为Hypersil BDS C18 (4.6mm×200mm,5μm),流动相为乙腈-水(42∶58),柱温25℃,流速1.0mL·min -1,检测波长为210nm.结果:枳实、枳壳中柠檬苦素含量测定方法的线性关系良好,线性范围和相关系数为0.0202～5.05 μg(r=0.9999),回归方程为Y=709794X -5168.9；平均回收率为98.64％,RSD=1.08％ (n=6).结论:该方法精密度、重现性和分离效果好,分析快速准确,操作简便,可用于枳实、枳壳中柠檬苦素的含量测定.枳实中柠檬苦素含量总体上高于枳壳,不同产地枳实、枳壳中柠檬苦素含量差别较大.%Objective: To establish the method of detecting the limonin in fructus aurantii immaturus and fructus auran-tii; Determine and compare the content of limonin in fructus aurantii immaturus and fructus aurantii from different areas. Methods; HPLC was applied. The column was Hypersil BDS C18 (4. 6mm ×200mm, 5μm) , the mobile phase was Acetonitrile: H2O =42 : 58, the temperature of column was 25 ℃ , the flow rate was 1. 0mL · min-1 , the detection wavelength was 210nm. Results; The method of determination limonin in fructus aurantii immaturus and fructus aurantii had good linear relationship. The linearity of limonin was in the range of 0. 0202 -5. 05μg (r =0. 9999) ; regression equation was Y =709794X-5168. 9; the average recovery of limonin was 98. 64% (RSD = 1.08%, n=6). Conclusion; The method has good precision 、 reproducibility and separation. It is simple, quick and reliable. It can be used for the determination of limonin in fructus aurantii immaturus and fructus aurantii. The content of limonin in fructus aurantii immaturus was generally higher than that of fructus aurantii and the content varied greatly among different areas.