低聚壳聚糖对中华绒螯蟹免疫功能的影响

摘要

A 28-day feeding trial was conducted to study the effect of diets containing different level of chi-tooligosaccharide(0‰,0.05‰,0.1‰,0.2‰,0.4‰)on immune function of Chinese mitten-handed crabs(Eriocheir sinen-sis) with initial mean weight of (15.78 ± 3.21)g.The immune indices of haemolymph was determined after 14 and 28 days of feeding.The results showed that:Chitooligosaccharide could significantly improve the activities of respiratory burst , serum phenoloxidase,superoxide dismutase,lysozyme,and catalase (P<0.05).Consequently,the immunoprotection rate of E.sinensis was enhanced.which of each adding group were 12.5%,37.5%,62.5%,50%,respectively,and 200 mg adding group was the nighest.The suitable dosage of chitooligosaccharide was 0.2‰.%为探讨低聚壳聚糖对中华绒螯蟹免疫功能的影响，在基础饲料中分别添加0.05‰、0.1‰、0.2‰、0.4‰的低聚壳聚糖，对均重为（15.78±3.21）g 的中华绒螯蟹进行为期28 d的饲养试验。在试验开始后第14、28天进行血液免疫学指标测定，结果表明，饲料中添加一定水平的低聚壳聚糖能够显著提高河蟹呼吸爆发活性，血清酚氧化酶、超氧化物歧化酶、溶菌酶和过氧化氢酶的活性（P＜0.05）。河蟹的免疫保护率明显提高，各添加组的免疫保护率分别为12.5%、37.5%、62.5%、50%，其中200 mg添加组最高。综合考虑，低聚壳聚糖的适宜添加量为0.2‰。