The ultrasonic-assisted extraction conditions of total flavonoids from Sechium edule(Jacq.)Swartz were stud-ied.Single factors including alcohol concentration,ultrasonic temperature,the ratio of liquid to material and ultrasonic time were investigated.Response surface methodology with 4 factors and 3 levels followed by verification test was adopted and a second order quadratic equation was established.The results showed that the effect order of four factors on the total flavonoids yield was as follows:ultrasonic temperature,ultrasonic time,the ratio of liquid to material and alcohol concentration.The op-timum condition were alcohol concentration 69%,ultrasonic temperature 71℃,the ratio of liquid to material 25 mL/g and ul-trasonic time 31 min.Under the optimized conditions,the total flavonoids yield reached to 3.402 %,the relative error was 0.526% compared to the predictive value,which indicated the feasible model fitted well with the experimental data.Total flavonoids yield using ultrasonic-assisted extraction was 12.69%higher than that of traditional heat refluxing extraction.%本试验研究了超声辅助提取佛手瓜中总黄酮的工艺。在单因素试验基础上,考察乙醇浓度、超声温度、液料比和超声时间对佛手瓜总黄酮提取率的影响。根据Box-Benhnken中心组合试验设计原理,采用响应面法对佛手瓜总黄酮提取的关键参数进行了优化,建立了佛手瓜总黄酮提取率的数学模型。结果表明:4个因素对佛手瓜总黄酮提取率影响的大小顺序是超声温度>超声时间>液料比>乙醇浓度。佛手瓜总黄酮的最佳提取工艺条件为:乙醇浓度69%、超声温度71℃、液料比25 mL/g、超声时间31 min,在此条件下总黄酮提取率为3.402%,与理论预测值相对误差为0.526%。与传统热水浸提法相比,提取率提高了12.69%。
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