ASBR处理食品废水中DOM转化过程的荧光光谱

摘要

利用厌氧序批式反应器(ASBR)处理食品废水,结合三维荧光光谱技术考察不同反应时段废水中溶解性有机物(DOM)的光谱特征和物质来源,并建立DOM特征峰荧光强度与氨氮浓度的关系.工艺运行结果表明：食品废水经过ASBR处理后,进水的COD从1100mg/L降至91mg/L,COD去除率达到91.73%,说明ASBR反应器可有效降解食品废水中的有机物质.三维荧光光谱显示,5种物质的特征荧光峰,即高激发波长色氨酸(峰A)、低激发波长色氨酸(峰B)、可见光区富里酸(峰C)、紫外光区富里酸(峰D)、类腐殖酸(峰E).随着厌氧生物处理的进行,峰A、峰B和峰C的荧光强度表现为先增加后减少的趋势；峰D荧光强度表现为微弱增加趋势；峰E荧光强度为先减少后增加趋势.荧光光谱指数FI、HIX、BIX表明,废水具有明显生物源特征.建立高激发波长色氨酸、低激发波长色氨酸特征峰荧光强度与色氨酸荧光强度之和与氨氮浓度在反应周期内的相关性,其相关系数分别为0.8136、0.9390、0.9153,说明可通过三维荧光光谱技术快速监测食品废水厌氧生物处理过程中的氨氮浓度.%The spectral characteristic and the source of the dissolved organic matter (DOM) were investigated in the treatment of food wastewater with anaerobic sequencing batch reactor (ASBR) through three dimensional fluorescence spectra, and the correlation was analyzed between fluorescence intensity of DOM characteristic peak and ammonia nitrogen concentration. The ASBR results showed that the organic substances can be degraded effectively in food wastewater: the COD concentration in influent and effluent were 1000mg/L and 91mg/L respectively, and the removal rate of COD was 91.73%. There were fluorescence peaks of five substances in three-dimensional fluorescence spectra: high excitation wavelength tryptophan (peak A), low excitation wavelength tryptophan (peak B), visible light wavelength fulvic acid (peak C), UV light wavelength fulvic acid (peak D), and humic acid (peak E). During the reaction, the fluorescence intensities of peak A, peak B and peak C first increased but afterwards decreased, the fluorescence intensity of peak D showed a weak increasing trend, and the fluorescence intensity of peak E first decreased and afterwards increased. Fluorescence spectrum parameters, including fluorescence index (FI), humification index (HIX), and biological index (BIX), indicated that wastewater obviously has biological characteristics. Statistical analyses pointed to correlations between ammonia nitrogen and fluorescence intensity of tryptophan, including fluorescence intensity of peak A, fluorescence intensity of peak B, sum of tryptophan fluorescence intensity, with correlation coefficients being respectively 0.8136, 0.9390 and 0.9153. In the anaerobic biological treatment of food wastewater, rapid detection of ammonia nitrogen can be realized through three-dimensional fluorescence spectrometry.