不同前处理方法对饲草中黄曲霉毒素B1测定的影响

摘要

本试验以样品加标回收率为指标,比较了液-液萃取、直接提取、亲和层析三种前处理方法对饲草中黄曲霉毒素B1测定值准确性的影响.应用酶联免疫法(ELISA)进行测定,并通过液相色谱荧光检测法(HPLC)进行验证.结果,采用液-液萃取-ELISA法,平均回收率在70.0％～102.0％,且相对标准偏差(RSD)＜6.6％,亲和层析净化后ELISA与HPLC结果基本一致,但较液-液萃取测定值低.提示液-液萃取法作为ELISA检测饲草中黄曲霉毒素B1的前处理方法为最佳方法.%To determine the best pretreatment of aflatoxin B1 in forage by ELISA,this paper compared liquidliquid extraction,direct extraction and immunoaffinity chromatography with standard recovery rate of samples,and then verified by HPLC with fluorescence detection.As for liquid-liquid extraction,the average recovery rate is 70.0％～102.0％,and relative standard deviation(RSD) is less than 6.6％.ELISA is roughly identical to the rusults of HPLC,but it is lower than liquid-liquid extraction.The result showed that,it is feasible and accurate to determine AFB1 by liquid-liquid extraction pretreatment method in forage.