花椒产香内生真菌的分离鉴定及其挥发性成分分析

摘要

Twelve strains of endophyte are purified from fresh Pericarpium Zanthoxyli.One of them is selected as a sample because of its fragrant metabolite and speedy growth.The metabolite analyzed by GC-MS shows that the fragrant components are mainly from 1-Octadecene(8.79％),Pyrrolo[1,2-a]pyrazine-1,4-dione,hexahydro-(6.79％),Bicyclo[2.2.2]octane-1-carboxylic acid(6.24％),2-Buta-none,3-hydroxy-(4.67％),Pyrrolo [1,2-a] pyrazine-1,4-dione,hexahydro-3-(phenylmethyl)-(4.10 ％),Pyrrolo[1,2-a] pyrazine-1,4-dione,hexahydro-3-(2-methylpropyl)-(3.83 ％),5-Eicosene,(E)-(3.72 ％),5-Isopropylidene-3,3-dimet hyl-dihydrofuran-2-one (3.54 ％),alpha.-Santalol (3.27 ％),Dodecanoic acid(3.25 ％),1,2-Benzenedicarboxylic acid,butyl 2-methylpropyl ester (2.97 ％),1,2-Benzenedicarboxylic acid,diisooctyl ester (2.82％).%从新鲜的花椒茎中分离内生真菌并进行分离纯化,共获得12株纯系微生物,通过筛选获得1株产香浓郁的菌株,利用GC-MS对其发酵产物进行分析.结果表明:该菌株代谢产物的香气成分主要为其中1(2)号菌株检出的61种化合物,菌株1(2)发酵液挥发性成分主要为十八烯(8.79％)、六氢吡咯并[1,2-a]吡嗪-1,4-二酮(6.79％)、双环[2.2.2]辛烷-1-羧酸(6.24％)、3-羟基-2丁酮(4.67％)、[1,2-a]吡嗪-1,4-二羰基-3-苯甲基-萘吲哚(4.10％)、3-(2-甲基)六氢吡咯并[1,2-a]吡嗪-1,4-二酮(3.83％)、5-二十碳烯(3.72％)、5-异亚丙基-3,3-二甲基二氢呋喃(3.54％)、2-氨基-丙二醇(3.27％)、月桂酸(3.25％)、1,2-苯二甲酸丁基二甲基酯(2.97％)、邻苯二甲酸二异丁酯(2.82％).