超声提取葡萄籽原花青素工艺的优化及其抗氧化活性研究

摘要

Response surface methodology (RSM)is used to optimize the ultrasonic extraction technology of procyanidins from grape seeds. On the basis of single factor test, a central composite design experiment is conducted and the response surface analysis is employed to investigate the influences of four main factors including ultrasonic extraction temperature,ratio of solvent to raw material,ethanol concentration and ultrasonic time on the yield of procyanidins.Then a regression model is established. The optimum condition is temperature of 55 ℃,ratio of solvent to raw material of 20(mL/g),ethanol concentration of 65% and extraction time of 10 min.Under such conditions,the yield of procyanidins is 2.482%,which is extremely consistent with the experimental value with a relative error of 0.36%. Pyrogallol oxidation method is applied for determination of antioxidant activities of procyanidins in grape seeds.The results show that the extraction has capacity to scavenge radical and the scavenging activity increases with the procyanidins concentration.%研究利用响应曲面法优化超声提取葡萄籽原花青素的工艺。在单因素实验基础上，采用中心组合设计响应面实验，考察了提取温度、液料比、乙醇浓度以及超声时间对原花青素提取率的影响，并建立回归模型。优化后的工艺：提取温度55℃，液料比20（mL/g），乙醇浓度65％，超声时间10 min；在此条件下葡萄籽原花青素的提取率为2．482％，与回归模型预测值的相对偏差为0．36％。同时进行了提取物的抗氧化性检验，结果表明葡萄籽原花青素对超氧阴离子自由基具有较好的清除能力，且呈剂量相关性。