高效液相色谱法测定月饼中的脱氢乙酸、苯甲酸、山梨酸、糖精钠、安赛蜜

摘要

本文建立了高效液相色谱测定月饼中脱氢乙酸、苯甲酸、山梨酸、糖精钠、安赛蜜的五种食品添加剂方法,验采用ZORBAX Eclipse XDB-C18色谱柱(4.6×250mm,5μm),以0.02mol/L的乙酸铵-甲醇(96∶4)为流动相,柱温30℃,二极管阵列检测器在波长230nm进行检测.采用外标法进行定量分析.结果显示：脱氢乙酸、苯甲酸、山梨酸、糖精钠、安赛蜜的线性良好,检出限分别为4.0、0.5、0.5、0.5、2.0 mg/kg,定量限分别为5.0、1.8、1.2、3.0、4.0 mg/kg.对月饼样品进行加标回收,平均回收率均在96.6~99.2%之间,6次测定的相对标准偏差均小于3%,该方法具有准确、快速、简单以及灵敏度高的特点,能够适应大规模样品的快速分析要求.%　　This paper reported the methods for determination of Determination of dehydroacetic acid (sodium),benzoic acid (sodium), sor-bic acid (potassium), saccharin sodium and acesulfame-K in moon cake by High-efficiency Liquid Chromatography. The separation was utilized ZORBAX Eclipse XDB-C18 column(4.6 ×250mm, 5μm) with 0.02mol/L NH4Ac-methanol(96 ∶4)as mobile phase and column temperature was 30℃,the experiment had been detected at 230nm through a photo-diode array detector. The contents were determined by external standard method for quantitative analysis. The results showed that the working curves for five kinds of food additives were good linearity, and the method detection limits were 4.0、0.5、0.5、0.5、2.0 mg/kg, and quantization limits were 5.0、1.8、1.2、3.0、4.0mg/kg. The recovery rates were 96.6%~99.2%,and 6 parallel determination of the relative standard deviation is less than 3%. This method is rapid, simple and accurate, high sensitivi-ty for measurements of benzene homologues. Its can adapt to quick analysis the large samples.