信阳水牛GHRH基因第二外显子的SNPs检测

摘要

[Objective]This study was aimed to detect the SNP of GHRH gene. [Method]The Genomic DNA was taken from 56 Xinyang buffalo blood samples. PCR primers were designed according the sequence of GHRH gene （gi. 194719389）. PCR-SSCP and DNA sequencing methods were used to detect the mutations. [Results]The results showed that there were six different band types in different individuals by PCR-SSCP analysis. 8 SNPs were detected, located at 4461bp （C→T mutation）,4 529 bp （T→G muta tion）,4 845 bp （G→A mutation）,5 053 bp （G→A mutation）,5 058 bp （G→T mutation）,respectively. [Conclusion]The results indicated that GHRH gene was polymorphic in Xinyang buffalo population%[目的]检测信阳水牛群体GHRH基因的SNP多态性。[方法]以56头信阳水牛为材料,采集血样,提取基因组DNA,以GenBank公布的牛GHRH基因（gi：194719389）序列作为参照序列,设计PCR引物,采用PCR-SSCP和DNA测序方法对该基因第二外显子的进行突变检测。[结果]经SSCP分析发现,这些个体总共出现六种不同的带型。测序结果表明：位于GHRH基因的4 461bp、4529bp、4 845bp、5 053bp和5 058bp处分别存在C→T、T→G、G→A、G→A和G→T五个单核苷酸突变。[结论]表明该基因在信阳水牛群体中单核苷酸多态较为丰富。