一株产中温淀粉酶芽孢杆菌的培养条件优化

摘要

从土壤中分离到1株产淀粉酶的芽孢杆菌,通过单因素和正交试验对其产酶条件进行了优化.结果表明,该菌株在1.5％玉米淀粉、1％牛肉膏、0.15％硫酸镁、0.1％氯化钙培养基中,接种量为2％ (v/v)、初始pH值为7.0、装液量10％、发酵温度37℃、180r/min振荡培养24h时酶活力最高,其酶活达到了2155.45U/mL.%The α-amylase-producing conditions of a Bacillus sp., which was isolated from soil, were optimized by single factor and orthogonal experiments. The results showed that when 2%(v/v) Bacillus sp. Was inoculated in the medium containing 1.5% corn starch, 1% beef extract, 0.15% MgSO4 and 0.1%CaCl2at the volumn of 10% and cultivated at initial pH 7.0 and 37℃ with a shaking speed of 180r/min for 24h, the activity of a-amylase could reach 2155.45U/mL.