Isolation and Cultural Conditions Optimization for Poly alpha-Hydroxybutyrate Producing Bacillus Species

摘要

The amount of plastic waste increases every year and exact time for its degradation is unknown. Out of a total of 300 isolated strains, one strain was selected for PHB production in different conditions like carbon source, nitrogen source, and incubation temperature and time. When PHB production conditions were optimized with different carbon and nitrogen sources, the highest PHB production was observed with maltose and ammonium sulphate. Regarding incubation time and temperature and pH, optimum PHB production conditions were 72 h, 40 degrees C and 7.0, respectively. The isolate was characterised biochemically as Bacillus species. The present study provide the useful data about the optimized conditions for PHB production by Bacillus species that can be utilized for industrial production of PHB, a fast emerging alternative of non-biodegradable plastics.