金线莲α-葡萄糖苷酶抑制剂提取工艺优化研究

摘要

Using Anoectochilus roxburghii as raw material,α-glucosidase inhibitor was extracted from A.roxburghii by ultrasonic-assisted extraction method.The effects of extraction temperature,time,solid-liquid ratio and extraction times on the inhibition rate of α-glucosidase were investigated.On the basis of single factor experiments results,the extraction process conditions were optimized by orthogonal experiments.The optimum extraction conditions were determined as:extraction temperature 60 ℃,time 90 min,solid-liquid ratio 1:15 (g:ml) and extraction time 1.Under the conditions,the inhibition rate ofA.roxburghii extracting solution on α-glucosidase was 31.98％.%以金线莲为原材料,采用超声辅助提取法提取金线莲α-葡萄糖苷酶抑制剂,考察提取温度、提取时间、固液比、提取次数对α-葡萄糖苷酶抑制率的影响.在单因素试验结果的基础上,采用正交试验对提取工艺条件进行优化.确定最佳提取工艺条件为提取温度60℃,提取时间90 min,固液比1:15 (g:mL),提取次数为1次.在此工艺条件下,所得的金线莲提取液对α-葡萄糖苷酶的抑制率为31.98％.