HPLC法同时测定葡萄和葡萄酒中6种基本花色苷

摘要

The method for simultaneous determination of six kinds of basic anthocyanins in grape and wine by HPLC was established.The simple was extracted by ethanol/hydrochloric acid/water (2∶1∶1,V/V),separated by Agilent-ZORBAX SB-C18 chromatographic column (250 mm×4.6 mm,5 μm),with acetonitrile and 0.1％ phosphoric acid solution as mobile phase,flow rate 0.8 ml/min,and detection wavelength 525 nm.Under the chromatographic conditions,the six anthocyanins got good separation effect within 30 min,and the contents showed a good linear relation with the peak area (R＞0.999 3),recovery rate was 82.2％-93.3％ and the precision was good (RSD＜5.0％).The method was convenient and fast,which could realize qualitative and quantitative detection of the six anthocyanins in grape and wine.%该研究建立了高效液相色谱法同时测定葡萄和葡萄酒中6种基本花色苷的检测方法.样品经无水乙醇/盐酸/水(2∶1∶1,V/V)提取,采用Agilent-ZORBAX SB-C18色谱柱(250 mm×4.6 mm,5 μm)为固定相,流动相A(乙腈)和B(0.1％磷酸水溶液)梯度洗脱,流速0.8 mL/min,检测波长525 nm.在该色谱条件下,6种花色苷在30 min内得到了很好的分离效果,其含量与峰面积呈现良好线性关系(R＞ 0.999 3),且回收率为82.2％～93.3％、精密度好(RSD＜5.0％).该方法方便、快速,能够实现对葡萄和葡萄酒中6种花色苷物质的定性及定量检测.