固态发酵玉米黄粉饲料复合菌种的筛选

摘要

The mixed strains for fermented corn gluten meal feed were screened by dot-inoculating,dot-inoculating stimulus circle method and multi-strains solid-state fermentation.The results showed that the three strains of Bacillus subtilis,Bacillus coagulans and Bacillus licheniformis were mixed by 1∶1∶1 with inoculum 5％ in the fermentation medium,the fermentation effect was better.Under the conditions of 30 ℃ for fermentation 120 h,the soluble protein content in corn gluten meal feed increased from 3.78％ to 14.26％.%运用平板点种法、点种刺激圈法和多菌种固体发酵法3种主要方法,筛选发酵玉米黄粉饲料的复合菌株.结果表明,枯草芽孢杆菌(Bacillus subtilis)、凝结芽孢杆菌(Bacillus coagulans)和地衣芽孢杆菌(Bacillus lichcniformis)3种菌株以1∶1∶1的比例混合,按5％的接种量接入发酵培养基,发酵效果较好,于30℃条件下发酵120 h,玉米黄粉饲料中可溶性蛋白含量由3.78％提高到14.26％.