定量检测马动脉炎病毒方法的建立

摘要

为建立快速、敏感、准确的马动脉炎病毒检测方法,笔者选取病毒基因组中高度保守的ORF7序列设计引物和Taq-Man探针,分别使用马动脉炎病毒的总RNA和含有选定检测序列的克隆标准品进行一步法实时定量RT-PCR,绘制扩增曲线,两曲线斜率之差小于0.1,证明两者的扩增效率相同,可用选定检测序列的克隆标准品对马动脉炎病毒进行定量检测.%A one-step fluorogenic RT-PCR(5'-nuelease probe-based) assay using a primer/TaqMan probe set designed a-gainst a highly conserved region of open reading frame 7 (ORF7) of EAV genome was developed for the specific detection of e-quine arteritis virus. This assay is sensitive, fast and exact. Customs and departments of quarantine can fast and exactly examine equine arteritis virus by this assay.