VEGF-A、VEGF-C及PLGF基因在奶牛乳腺发育各时期的表达变化

摘要

试验旨在研究奶牛VEGF-A、VEGF-C及PLGF基因在乳腺发育各个时期的mRNA表达情况,进而为下一步研究奶牛乳腺发育功能基因奠定基础.采用Real-time PCR SYBR Green I染料法,所得结果与Affymetrix公司的基因芯片差异筛选结果作比较分析.结果表明,3个基因在乳腺发育11个时期均有表达.VEGF-A基因在妊娠后期及泌乳期表达量较高且与其它时期差异显著(P＜0.05);VEGF-C基因在泌乳期表达量较高；PLGF基因的表达量从青春期开始到妊娠期呈递增趋势,到妊娠期4个月达到最高表达量,其后表达量逐渐递减.从以上结果可以得出,VEGF-A及VEGF-C基因可能在奶牛泌乳中发挥作用,而PLGF基因对奶牛乳腺发育起到重要作用,因此通过PCR扩增出PLGF基因的全部CDS序列,为下一步研究PLGF基因功能奠定基础.%In order to study the mRNA expression changes of VEGF-A ,VEGF-C,and PLGF gene in different developmental periods of dairy cow mammol/lary gland, then lay the foundation for further research on the gene function of the cow mammol/lary gland. The SYBR Green I dye Real-time PCR method was used,and the results were compared, with gene chip differential screening results of Affymetrix company. The results showed that three genes were expressed in 11 periods of mammol/lary gland. VEGF-A gene expression during late pregnancy and lactation was higher and significantly different from other periods(P<0. 05);VEGF-C gene expression was higher during lactation; PLGF gene expression from the onset of virgin to pregnancy increases gradually to 4 months pregnancy the highest expression level, followed by gradually decreasing the expression. The results could be drawn from the above, VEGF-A and VEGF-C gene may play a role in the lactating cows, and PLGF gene might be play an important role to cow mammol/lary gland development, so get the PLGF CDS sequence by PCR amplified method, lay the test foundation for further research on the gene function of PLGF gene.