棉粕源生物蛋白质饲料底物微量元素添加量的优化

摘要

本研究的目的是优化棉粕源生物蛋白质饲料底物微量元素的添加量.试验采用5因子6水平均匀设计(uniform design)法,通过测定发酵产物中游离棉酚(free gossypol,FG)含量和活性酵母菌数,优化热带假丝酵母(Candida tropicalisZD-3)和酿酒酵母(Saccharomyces cerevisiae ZD5)对棉粕源基础底物复合发酵的底物微量元素的添加量.应用DPS v7.05统计软件分析试验数据,建立回归模型,获得优化结果.底物微量元素添加量的优化结果为硫酸亚铁540 mg/kg,硫酸铜4mg/kg,硫酸锰180 mg/kg,硫酸锌15 mg/kg,亚硒酸钠0 mg/kg.%The purpose of this study was to optimize the microelement additive amount in substrate for biological protein feed from cottonseed meal during solid state fermentation. The uniform design of 5 factors and 6 levels was applied, by measuring the free gossypol and yeast content of fermented product, to optimize the microelement additive amount in medium during solid state fermentation with Candida tropicalis ZD-3 and Saccharomyces cerevisiae ZD-5. The experimental data was analyzed to obtain the regression model and the optimal supplementary material composition was achieved by optimization with DPS v7.05 software. The optimal microelement additive amount consisted of 540 mg/kg FeSO4, 4 mg/kg CuSO4, 180 mg/kg MnSO4, 15 mg/kg ZnSO4, 0 mg/kg Na2SeO3.