This experiment was to study the effect of the polymorphisms in the Leptin gene and SCD1 gene. By direct sequencing of PCR products, 7 SNPs were found in the Leptin gene exons 2 and 3, of which four were non-synonymous mutations; 1 SNP was found in the SCD1 gene exon 5, which was a non-synonymous mutation. Allelic substitution effect of all the SNPs on 25 single fatty acids, monounsaturated and polyunsaturated and desaturation indexes were tested. The results showed that both the SCD1 SNP and 3 SNPs of the Leptin gene affected the desaturation of FA into MUFA to different extents. It was reported that one of the roles of Leptin in the metabolism was to down regulate the transcription level of SCD1 gene, so, it possible that the non-synonymous mutations in the Leptin gene also contributed to the variability of FA composition in muscle fat except for the mere additive effect of SCD1 gene on FA desaturation.%试验旨在研究Leptin和SCD1基因多态性对天祝白牦牛肌肉脂肪酸含量的影响.通过对PCR产物直接测序,在Leptin基因外显子2和3共发现7个SNPs位点(其中4个是错义突变);在SCD1基因第5外显子区发现1个SNP位点(错义突变),对所有SNPs等位基因替代效应对25种单一脂肪酸、单一不饱和度指数、多不饱和度指数和去饱和效应指数的影响进行了检测.结果发现,SCD1基因SNP和Leptin基因3个SNPs位点在不同程度上影响到由饱和脂肪酸FA到单一不饱和脂肪酸MUFA的去饱和作用.说明除了SCD1基因对FA去饱和作用的影响外,Leptin基因错义突变也会对肌肉脂肪中FA的组成产生影响.
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