新城疫流行株GX-08全基因序列分析及其对鸭的致病性研究

摘要

2008年从患病免疫鸡群中分离到1株新城疫病毒(Newcastle disease virus,NDV)流行株,经致病指数测定为强毒株,编号为GX 08.致病性试验表明,GX-08株对鸭具有感染性和致病性.参照GenBank发表的NDV基因序列设计10对特异性引物,采用RT-PCR方法对GX-08株全基因组序列分段进行扩增.拼接后序列分析表明GX-08株的全基因组序列总长为15192 nt,包含6个开放阅读框,分别编码6种蛋白质NP、P、M、F、HN和L.F基因裂解位点处的氨基酸序列为112 R-R-Q-K-R-F(117),符合强毒株氨基酸序列特点.F基因第47-420位片段基因分型分析结果表明,该流行株属于基因Ⅶ型.%Newcastle disease virus strain GX-08,isolated from the invasive immunized chicken flocks in Guangxi province in 2008, was velogenic according to the pathogenetic tests and highly pathogenicity to duck. According to the sequences of NDV strains published in GenBank,ten pairs of primers were designed and complete genome cDNA of the isolate was successfully amplified by PT-PCR. Sequence analysis showed that the genome length of GX-08 strain was 15192 nt which contained six ORFs,encoding 6 proteins of NP, P, M, F, HN and L, respectively. The cleavage motif 112 R-R-Q-K-R-F117 of F protein was identical to the characteristic of velogenic strain. The evolution analysis of the 47 to 420 nt sequence including the cleavage site of F gene indicated that GX-08 strain belonged to genotype Ⅶ.