DNA resequencing of the H-FABP gene intron 2 in 16 pigs from 9 breeds which were Zang pig, Exi pig,Large White pig, Laiwu Black pig, Beijing Black pig, Wild pig, Rongchang pig, Bamei pig and Licha Black pig respectively revealed 14 polymorphisms in 2078 bp. Hudson-Kreitman-Aguade's results showed that a nucleotide diversity(π) of south west type pigs,north type pigs, deloped and imported breeds and all breeds were 0. 18％, 0. 25％, 0. 29％, 0. 25％, respectively; the Tajima's D value of south west type pigs, north type pigs and all breeds were -0.52, 0.76 and -0. 60, respectively; the Fu and Li's D value of south west type pigs, north type pigs and all breeds were -0.30, 0.72 and -0.17, respectively. This results indicated that the SNPs of H-FABP intron 2 were very polymorphic in different breeds, and also, the Neighbour-Joining trees of their haplotypes did not show a geographical arrangement for any of the genes, we inferred some loci which may infect intra muscular fat(IMF).%本研究以藏猪、鄂西黑猪、大白猪、莱芜黑猪、北京黑猪、野猪、荣昌猪、八眉猪和里岔黑猪等国内外9个品种16头猪为研究对象,应用Primer Premier 5.0软件设计引物对心脏脂肪酸结合蛋白(heart fatty acid binding protein,H-FABP)基因第2内含子进行扩增测序后拼接,获得了长2078 bp的第2内含子全序列,发现了14个位点的SNPs;对所有突变进行Hudson-Kreitman-Aguade测验等遗传分布分析结果发现,西南型、高原型猪核酸分布值(π)、Tajima's D测验值(DT)、Fu和Li's D测验值(DFu and Li)分别为0.18%、-O.52和-O.30,华北型猪π、Dr和DFu and Li分别为0.25%、0.76和0.72,培育和国外品种猪π值为0.29%;对所有类型的猪进行分析,其π、Dr和DFu and Li分别为0.25%、-0.60和-0.17,在不同的猪种中各个位点的SNPs变异很大,对其进行构建进化树等一系列分析,得到相似结论,并最终推断出可能会影响肌内脂肪(intra muscular fat,IMF)含量的突变位点,为研究H-FABP与IMF的关系提供了理论基础.
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