猪H-FABP基因内含子1的一个遗传多态性及其遗传效应分析

摘要

[目的]为H-FABP基因运用于猪育种过程中的标记辅助选择提供基础资料.[方法]应用PCR-SSCP方法分析H-FABP基因在山西白猪、马身猪、大白猪、长白猪和杜洛克猪5个猪种的多态性,并研究基因型与肌内脂肪含量的相关性.[结果]在猪H-FABP基因内含子1扩增的片段上发现了一个多态性,检测到2个等住基因(A、B)、3种基因型(AA、AB、BB),并对纯合子进行测序,发现SSCP的变异是由碱基C→T的替换造成的.基因型在不同猪种分布的多重比较表明,马身猪与山西白猪、长白猪、大白猪、杜洛克猪间基因型分布盖异极显著(P＜0.01),其他纯种猪群间基因型分布差异不显著(P＞0.05).固定效应模型分析表明,肌内脂肪舍量基因型间差异极显著(P＜0.01).最小二乘分析表明,BB基因型个体与AA基因型个体比较肌内脂肪含量差异显著(P＜0.05).[结论]H-FABP基因对猪肉品质存在一定的影响.%[Objective] The aim was to provide basic reference for the use of H-FABP gene in marker-assisted selection of the breeding process of pig. [Method] Single-nucleotide polymorphisms of the H-FABP gene in Shanxi White pig,Mashen pig,Large White pig.Landrace and Duroc were tested by PCR-SSCP, and the correlation between genotype and intramuscular fat content in pigs were analyzed. [ Result] One polymorphism was found in the amplified region of intron 1 of porcine H-FABP gene,in which two alleles (A and 5) and three genotypes( AA, AB, and SB) were examined. C→T transition was detected by sequencing the homozygotes. The multiple comparison of the distribution of genotype in different pig varieties revealed that Mashen pig showed extremely significant difference ( P＜0. 01) in genotype distribution with Shanxi White, Landrace, Large White and Duroc breeds; whereas no significant differences (P＞0.05) were found in genotype distribution between other breeds. Based on the fixed effect model,extremely significant differences (P＜0.01) were found in the intramuscular fat content among different H-FABP genotypes. Using least square analysis,it was found that there was significant differences (P ＜0.05) in the intramuscular fat content between the individuals of the BB genotypes and those of the AA genotypes. [ Conclusion] The H-FABP genotype had significant effects on the meat quality.