影响Fat-1转基因水牛胚胎发育因素的研究

摘要

The effects of injecting timing after fertilization (5 to 6, 9 to 10, 13 to 14, 17 to 18, 21 to 22 h) and exogenous DNA concentrations (10, 50 and 100 μg/mL) on the development of Fat-1 transgenic embryo were investigated. The results showed that the EGFP expressing rate and blastocysts' EGFF expressing rate of 9 to 10 h group were the highest, the EGFP expressing rate were significantly higher than 5 to 6 h group(P<0. 01) and the blastocysts' EGFP expressing rate significantly higher than 21 to 22 h group(P<0. 01); in the concentration test, the EGFP expressing rate and blastocysts' EGFP expressing rate of 50 μg/mL group were the highest, and significantly higher than 100μg/mL(P<0. 01).%本试验比较了水牛卵母细胞体外受精后注射外源DNA的时间(5～6、9～10、13～14、17～18、21～22 h)和外源DNA的浓度(10、50、100 μg/mL)对Fat-1转基因水牛胚胎的发育影响.结果显示,9～10 h组的胚胎表达率和囊胚表达率最高,其中胚胎表达率极显著高于5～6 h组,囊胚表达率极显著高于21～22 h组(P＜0.01);在浓度试验中,50 μg/mL浓度组的胚胎表达率和囊胚表达率最高,且极显著高于100 μg/mL(P＜0.01).