健康中国恒河猴各淋巴组织中T淋巴细胞表型及分布

摘要

试验旨在明确T淋巴细胞在中国恒河猴各组织中的表型与分布,为疾病模型研究提供基础数据.取外周血、腹股沟淋巴结、肠系膜淋巴结及肠道组织,从中分离出淋巴细胞.使用流式细胞术检测分析各种表型的淋巴细胞在组织间的分布.结果表明,淋巴结中CD4+/CD8+T细胞比值高于外周血,肠道固有层中最低,三者差异显著.记忆性T细胞在外周血和肠道固有层T细胞中比重较大,而淋巴结中主要为幼稚T细胞.CD4+T细胞中中心记忆T细胞Tcm为主要亚群,而CD8+T细胞主要为效应记忆细胞Tem.外周血与肠道固有层中增殖T细胞比例相当,而淋巴结中T细胞增殖水平相对较低.各组织中CXCR4受体表达量普遍高于CCR5受体,其中肠道固有层CCR5受体表达水平最高.值得注意的是,有一小群表型CD3+ CD4+ CD8l0w的细胞仅在肠道固有层中存在,经分析其功能活性应高于肠道CD4单阳性T细胞.因此,测定了健康中国恒河猴各表型T淋巴细胞在多种淋巴组织中的基础数值,为相关模型研究奠定基础.%To quantitate and phenotype the T lymphocytes in a variety of lymphoid tissue in healthy Chinese-origin rhesus macaques. Lymphocyte composition of peripheral blood (PBL), inguinal lymph nodes (ILN), mesenteric lymph nodes (MLN), intestinal lamina propria (LP) from healthy rhesus monkeys was characterized in detail by flow cytometry. The results showed that CD4+ /CD8+ ratio in LN showed higher than that in the PBL and lowest in LP, there were significant differences among them. In the LN,most CD4 + T cells were naive T cells while in the PBL and LP most CD4+ T cells were memory T cells. In all lymphoid tissues,memory markers were Tcm from the vast majority of CD4+ T cells,but most of the CD8+ T cells expressed a memory phenotype of Tern. The population of proliferating CD4+ and CD8+ T cell was similar from both PBL and LP,and higher than that from LN. The chemokine receptors CXCR4 were expressed higher than CCR5 in all lymphoid tissues. 28% of CD4+ T cells in LP expressing the CCR5 that was the highest among all tissues. It was notable that there is small population of CD3+ CD4+ CD81ow cells exist only in LP. The results provides a basic reference values for further research related to immunity or AIDS model by using macaques.