不同繁殖季节成年牦牛附睾组织特征比较

摘要

为比较不同繁殖季节成年牦牛附睾组织结构特征变化，应用苏木精—曙红常规染色、Masson’s 和 Gomo-ri’s 特殊组织化学染色方法比较不同繁殖季节牦牛(6头繁殖期成年牦牛和9头繁殖间期成年牦牛)附睾的组织结构特点并用 IPP 图像分析软件进行定量分析。结果显示，与繁殖间期相比，繁殖期附睾尾间质胶原纤维和网状纤维较附睾头及附睾体明显增多；附睾头和附睾体柱状上皮厚度显著增加(P ＜0.05)，附睾头纤毛长度增加显著(P ＜0.05)；附睾头和附睾尾管腔内径及外径显著增高(P ＜0.05)；但是附睾尾管腔外径繁殖期与繁殖间期相比差异不显著(P >0.05)。本研究结果表明，成年牦牛附睾管外围网状纤维与胶原纤维分布一致，二者在附睾尾较为丰富，可能与其较强的收缩能力及精子运输有关；柱状纤毛上皮高度及纤毛长度、管腔内径与外径的变化与其所处的不同繁殖季节密切相关，附睾管腔的膨大和回缩亦可能是高原地区季节性繁殖的哺乳动物中一种普遍存在的现象。%To compare the histological changes of the adult yak’s epididymis in different breeding seasons,six adult yak testis in breeding season and nine adult yak testis in breeding interval were collected for structure investigation by HE staining,Masson’s and Gomori ’s histochemistry methods,and IPP (Image-Pro Plus)statistics method was used to quantitative statistics.The re-sults showed that comparing with the adult yak in the breeding interval,the epididymis ducts of adult yak in the breeding season were covered with the columnar ciliated epithelium.The collagen and reticular fiber in cauda epididymis were obviously more abundant than caput and corpus epi-didymitis.And the thickness of columnar epithelium cells in caput and corpus epididymitis,the length of the cilia in caput,and also the internal and external lumen diameter of caput and corpus epididymitis were all significantly increased in the breeding season (P <0.05 ),but the external lumen diameter of the cauda epididymis had no significant differences (P >0.05).In conclusion, the research showed that the distribution of collagen and reticular fiber in adult yak’s epididymis interstitial were similar,and they were more rich in cauda epididymis,which might relate to the capacity and the sperm transport;The changes of the epithelial thickness,the length of cilia,the internal and external lumen diameter were close related to the different breeding seasons,and it might be a common phenomenon in plateau mammals that the enlargement and reduction of the epididymal duct in different breeding seasons.