马鹿 Zfx/Zfy 基因的克隆及序列分析

摘要

Specific primers were designed according to the mRNA sequence of Bos taurus Zfx/Zfy genes provided in GenBank.Zfx/Zfy sequences of Cervus elaphus were cloned and ana-lyzed.The results showed that the cloned Zfx gene sequence of Cervus elaphus was 2 1 15 bp (GenBank accession No:KP257294.1 )that encoded 696 amino acids,the cloned Zfy gene se-quence was 2 406 bp (GenBank accession No:KU041539)that encoded 801 amino acids.The phy-logenetic trees of Zfx and Zfy amino acid were obtained using the Neighbor-Joining method of DNAStar software.Analysis showed that Zfx of Cervus elaphus was closely related to Homo sa-piens ,compared with Bison,Pantholops hodgosoniiand ,Panthera tigris ,Felis catus ,Bos taurus , Canis lupus familiaris ,Ovis aries .Zfy of Cervus elaphus was closely related to Bos taurus ,Bi-son,Ovis aries ,Pantholops hodgosonii compared with others.Zfx/Zfy genes of Cervus elaphus were cloned and sequenced for the first time,and this research provided a foundation for further studying the structure and biological functions of the proteins.%根据 GenBank 中公布的牛 Zfx/Zfy 基因的 mRNA 序列,设计特异性引物,对马鹿 Zfx/Zfy 基因进行扩增,并对该序列进行分析。结果表明,克隆得到马鹿 Zfx 基因序列长为2115 bp,GenBank 登录号为 KP257294.1,编码696个氨基酸；Zfy 基因序列长为2406 bp,GenBank 登录号为 KU041539,编码801个氨基酸。依据 Zfx/Zfy 基因氨基酸序列构建进化树,分析表明,马鹿 Zfx 基因与人的亲缘关系较近,与聚为一类的野牛、藏羚羊、虎、家猫、家牛、家犬、绵羊的亲缘关系稍远；Zfy 基因与家牛、野牛、藏羚羊、绵羊的亲缘关系较近,与其他动物及人的亲缘关系较远。本研究首次克隆了马鹿 Zfx/Zfy 基因,为研究该基因蛋白质的结构和功能奠定了基础。