通过化学诱变和基于基因组 DNA 诱变的遗传重组技术,对乙醇工业酵母菌(Saccharomyces cerevisiae)的抑制物耐受性进行改造,获得了重组酿酒酵母HN-1-24,其抑制物耐受性能和发酵性能均得到了提高. 重组菌株在含7g/L乙酸和1g/L糠醛的抑制物耐受性发酵培养基中,具有良好的糖转化率和乙醇产量,发酵100 g/L葡萄糖能够产生38 g/L乙醇. 在纤维素水解液发酵培养中进行乙醇发酵,发现重组菌株的乙醇发酵效率明显快于原始菌株,发酵时间提前6 h,且发酵终点乙醇产量为37 g/L,比原始菌株HN-1提高了8. 8%.%The recombinant Saccharomyces cerevisiae HN-1-24 was obtained by combination of chemical mutagenesis and genomic DNA mutagenesis-based genetic recombination methods to improve the inhibitor tolerance and fermentation performance. The recombinant strain HN-1-24 exhibited a high conversation rate of sugar to ethanol and ethanol production under a condition with 7 g/L acetic acid and 1 g/L furfural. Thus 38 g/L of ethanol could be obtained from 100 g/L of glucose. When ethanol fermentation of cellulose hydrolyzate culture was performed,the fermentation time of the recombinant strain was decreased by 6 h and the final ethanol concentration was enhanced by 8. 8% as compared with the original strain. The results of this study provided a basis for the large-scale production of fuel ethanol by using lignocellulose.
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