油橄榄果渣水溶性膳食纤维的组成成分和功能特性分析

摘要

The crude soluble dietary fiber( SDF) from Olive pomace( Olea europaea L. ) was obtained by using alkaline extraction method. The chemical composition, monosaccharide composition and functional groups were characterized by gas chromatography ( GC) and infrared spectrometry. Furthermore, the effects of olive pomace SDF on the in vitro NO2- adsorption and antioxidant activity were investigated. The results showed that the content of SDF in the crude olive pomace SDF was 60. 90% . The monosaccharide of crude SDF was composed of xylose, arabinose, rhamnose, glucose, galactose and mannose with the molar ratio of 64. 0:9. 4:3. 9:3. 9:2. 6:1. 6. The major component was xylose, followed by arabinose. Infrared spectra showed that the crude SDF had the characteristics absorption peaks of polysaccharide. The maximal adsorption capacity of SDF for NO2-in simulated gastric environment at pH=2(98. 92% ) was greater than that in the intestine environment at pH=7(8. 69% ). The maximum scavenging ratio of the crude SDF for hydroxyl radical(·OH) was 46. 34% at the mass concentration of 6 g/L and weaker than that of Vc. The crude SDF had strong DPPH radical scavenging activity with the IC50 of 0. 06 g/L. The DPPH radical scavenging activity of the crude SDF was weaker than that of Vc at low concentrations and close to that of Vc at high concentrations.%以碱法制备的油橄榄果渣水溶性膳食纤维(SDF)粗品为研究对象,通过化学分析、气相色谱和红外光谱等方法分析其化学成分、单糖组成和官能团组成,并测定其体外吸附NO2-及抗氧化活性等功能特性.结果表明:油橄榄果渣SDF粗品中含SDF为60.90％,其单糖主要由木糖、阿拉伯糖、鼠李糖、葡萄糖、半乳糖和甘露糖组成,其比例为:64.0:9.4:3.9:3.9:2.6:1.6,主要为木糖,其次为阿拉伯糖.红外光谱分析显示油橄榄果渣SDF粗品具有糖类的特征吸收峰;在体外模拟胃环境(pH值2)下,油橄榄果渣SDF对NO2-的最大吸附率达到98.92％,优于模拟肠环境(pH值7)下的8.69％;当SDF粗品质量浓度为6 g/L时,其对·OH的清除率达到最大为46.34％,弱于Vc;SDF粗品对DPPH·具有较强清除作用,其50％清除率对应的SDF粗品质量浓度(IC50)为0.06 g/L,其清除能力在低浓度时弱于Vc,在高浓度时接近Vc.