电喷雾质谱法研究不同碳链长度的全氟羧酸与肌红蛋白的相互作用

摘要

采用电喷雾质谱法（ESI-MS）检测了全氟丁酸、全氟己酸、全氟辛酸和全氟癸酸分别与肌红蛋白的相互作用，通过直接计算法测定了4种全氟羧酸与肌红蛋白的结合常数，并研究了碳链长度对全氟羧酸类化合物与肌红蛋白相互作用的影响。结果表明，不同碳链长度的全氟羧酸类化合物与肌红蛋白相互作用的能力有差别，碳链越长，其结合能力越强，且与蛋白质结合的配体数目越多。%The interactions between heptafluorobutyric acid,undecafluorohexanoic acid,perfluorooctanoic acid,nonadecafluorodecanoic acid with myoglobin were studied by electrospray ionization mass spectrometry (ESI-MS).The binding constants between four perfluoroalkyl acids and myoglobin were measured by direct cal-culation method.The effects of carbon chain length of perfluoroalkyl acids on their binding abilities to myoglo-bin were explored.The results showed that,the binding abilities of interactions between perfluoroalkyl acids with different carbon chain lengths and myoglobin were different,and the binding abilities and numbers of lig-and binding to myoglobin increased with the increasing of the carbon chain length.