荧光光谱法研究木犀草素及其苷与牛血清白蛋白的相互作用

摘要

In the Tris‐HCl buffer (pH=7 2.) ,the interaction of luteolin and luteolin‐7‐O‐β‐D‐glucoside with bovine serum albumin (BSA ) was studied by fluorescent spectrometry .The binding constants (Ka ) and the binding sites (n) were calculated .The results show that the combination of luteolin and luteolin‐7‐O ‐β‐D‐glucoside with BSA were strong and their fluo‐rescence quenching of BSA w as a static quenching process .%在pH＝72．的Tris‐HCl缓冲液中，利用荧光光谱法研究了木犀草素和木犀草素‐7‐O‐β‐D‐葡萄糖苷（木犀草苷）与牛血清白蛋白（BSA）分子间的相互作用，计算了二者与BSA的结合常数、结合位点数．研究表明，木犀草素和木犀草苷能够与BSA发生较强的相互作用，二者对BSA内源性荧光的猝灭机制属于静态猝灭．