本文采用甲醇、乙醇、乙酸乙酯和水4种溶剂提取黄香楝树皮中的活性成分,Folin-Ciocalteu法测定提取物中的总酚含量,甲醇、乙醇提取物中酚类活性成分含量较高,总酚含量分别为58．59、50．47mg·g-1。采用DPPH自由基清除法、邻二氮菲-Fe2+氧化法、过氧化氢清除法、还原力法、磷钼络合物法等5种抗氧化性测定方法研究提取物的抗氧化活性,乙醇提取物浓度为1．0mg·mL-1时,对 DPPH、·OH、H2 O2的清除率均大于90%,对DPPH、·OH、H2 O2的IC50值分别为234．2、555．2、60．1μg·mL-1。%The antioxidant activities of Hesperethusa crenulata bark extracts from methanol,ethanol,ethyl acetate and aqueous were investigated in this assay. The total polyphenolic contents of the extracts were evaluated,using the Folin-Ciocalteu method. The poly-phenolic contents of methanol and ethanol extracts were higher compared to ethyl acetate and aqueous extracts,which were 58. 59、50. 47mg·g-1 ,respectively. The antioxidant and free radical scavenging activities of extracts of the plant were also assessed against 2,2-diphenyl-1-picrylhydrazyl(DPPH),10-phenanthroline-Fe2+ oxidative assay of hydroxyl radical scavenging activity,hydrogen peroxide-scavenging activity,reducing power and phosphomolybdenum method. The results indicated that the scavenging abilities of ethanol extract on DPPH,·OH、H2 O2 were above 90%at the concentration of 1. 0mg·mL-1 . The IC50 of the ethanol extract on DPPH, ·OH,H2 O2 scavenging activities were 234. 2、555. 2、60. 1μg·mL-1 .
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