碱和酸碱处理对钛生物活性的影响

摘要

The titaniums were treated by alkali and acid-alkali to obtain TiO2 films with different morphologies and crystalline forms. The material morphologies were analyzed by scanning electron Microscopy( SEM) and the material crystalline forms were analyzed by X-ray diffraction(XRD). The samples were soaked in simulated body fluid(SBF)to observe the deposition of hydroxyapatite ( HA) on surface. The biological activity of samples were investigated by vitro cytotoxicity assays. The results showed that the titani-ums treated by alkali and acid-alkali had the same crystalline forms but different morphologies TiO2 thin films under the same heat treatment temperature. Comparison of the difference of biological activity,it showed that the titanium treated by acid-alkali at 450℃had a good biological activity,that attributed to its surface morphology with the small size flower-shaped nanowires. And it showed the titanium treated by alkali at 700 ℃ had an excellent biological activity,that attributed to its surface morphology of nanoparticles with small microscopic dimensions. So we concluded that the surface morphology on titanium has an important influence on its bio-logical activity.%利用碱处理和酸碱处理钛表面,获得不同形貌和不同晶型的TiO2薄膜。利用SEM对材料的表面形貌进行了分析,利用XRD测试了材料的晶型。将样品浸泡于模拟人体体液( SBF)中,观察材料表面羟基磷灰石( HA)的沉积情况,并进行体外细胞毒性实验,探讨其生物活性。结果表明：相同的热处理温度下,碱处理和酸碱处理的钛表面具有相同的晶型,但是表面形貌不同的TiO2薄膜。对比了其生物活性的差异发现：450℃的酸碱处理钛具有较好的生物活性,归于其表面有尺寸较小的花蕊状纳米丝形貌,700℃的碱处理钛具有优异的生物活性,归因于其表面具有较小微观尺寸的纳米颗粒形貌。