4,4'-二(对氨基苯乙炔基)-6,6'-二N,N-二(乙氧基羰甲基)氨甲基-2,2'-联吡啶的合成与表征

摘要

以4,4'-二硝基-2,2'-联吡啶-6,6'-二亚甲基双三氟乙酸酯作为起始原料,经水解、溴化、酯化和对氨基苯乙炔取代4步反应合成了4,4'-二.(对氨基苯乙炔基)-6,6'-二[N,N-二(乙氧基羰甲基)氨甲基]-2,2'-联吡啶.通过红外光谱、核磁共振波谱、高分辨质谱等表征了该化合物的结构.该化合物经水解后与铕离子形成稀土荧光螯合物,在紫外光激发下,发射出具有铕离子特征的荧光光谱.%In order to obtain a new solid phase time-resolved fluorescence immunoassay chelate, a new intermediate compound 4,4'- (p-aminophenylethynyl) -6,6' -bis [ N, N-bis ( ethoxycarbonylmethyl) amino methyl ] -2, 2'-bipyridine was prepared from4,4'-dinitro-2,2'-bipyridine-6,6'-dimethyl bis(trifluoroacetate) by hydrolyza-tion, bromination, esterification and substitution. The structure of the compound was charactered by DSC, IR, 'H NMR and HRMS. The compound was hydrolyzed in the solution of europium ion, forming a rare earth fluorescence chelate. The fluorescence spectrum of the chelate excited by an light source has peaks peculiar to the europium ion.