富精氨酸多肽修饰的金纳米粒子跨膜传输

摘要

考察了富精氨酸多肽功能化的金纳米粒子作为载体对细胞外物质的跨膜传输行为.通过生物素( Bio-tin)与亲和素(Strcptavidin)的亲和反应将具有特定跨膜功能的富精氨酸RRRRRRRR(R8)多肽分子连接到多肽CALNN修饰的金纳米粒子表面,实现粒子的功能化.以荧光素为模型化合物,利用激光共聚焦显微镜观察了纳米粒子的输送过程.实验结果表明,富精氨酸多肽功能化的金纳米粒子可以作为一种低毒高效的跨膜输送载体.%The transmembrane behavior of rich arginine( R) peptide modified gold nanoparticles was studied. The fiinctionalization of the gold nanoparticles was achieved by the conjugation of transmembrane peptide RRRRRRRR(R8) with peptide CALNN stabilized gold nanoparticle through the standard streptavidin-biotin chemistry. The transmembrane delivery of the model compound, fluorescein, was studied by confocal scanning laser microscope. The result suggests that the Rg modified gold nanoparticles can be used as an excellent vector for transporting extracellular materials into cells with low cellular cytotoxicity.