一种光功能有机纳米粒子的制备及细胞毒性评价

摘要

合成了一种具有双光子荧光探针功能的有机纳米粒子2,5,2’,5’-(4’-N,N-二苯胺苯乙烯基)联苯(DPA-TSB),并研究其细胞毒性.利用水溶性四氮唑(WST-1)法、乳酸脱氮酶(LDH)法和流式细胞术检测了胃癌细胞吞噬纳米粒子后的生理活性.研究结果表明,在纳米粒子浓度小于12 μg/mL时,胃癌细胞仍表现出较好的生理活性,表明该纳米粒子是一种具有较好生物安全性的光功能有机纳米粒子.%The cytotoxicity about organic nanoparticles which has the function of two-photon fluorescent probe 2,5 ,2',5'-tetra(4'-N,N-diphenylaminostyryl)biphenyl(DPA-TSB) was studied. The physiological activity of stomach cancer cells after englobing the nanoparticle was detected by the methods of WAT-1, Lactate dehydro-genase( LDH) and flow cytometry. The results show that the stomach cancer cells can live well when the concentration of the nanoparticles is below 12 μg/mL, which suggests that the nanoparticles have the property of good biological security.