单分子磁镊测量高亲和反应系数

摘要

介绍了一种基于单分子磁镊测量反应系数的分析方法,并以单链结合蛋白与单链DNA的结合过程为例,通过测量和计算,得出了相应的反应系数,并获得了反应的详细信息.%A method based on single molecule experiment was adopted. Single-molecule magnetic tweezers was used to study the different binding process of SSB ( E. coli single-stranded DNA binding protein) and ssDNA( single-stranded DNA ) in different conditions, and get the reaction rate and reaction detailed information. This method can be used in similar reactions.