通过动力学拆分方法，由3,5-双三氟甲基苯乙酮出发，经过 NaBH4还原，制备得到高纯度消旋化的(R,S)-3,5-双三氟甲基苯乙醇。经过筛选得到2种高效高选择性动力学拆分(R,S)-3,5-双三氟甲基苯乙醇的脂肪酶：Novozym 435和Rhizopus arrhizus。以Rhizopus arrhizus作为实验脂肪酶，考察了影响其动力学拆分的因素，包括溶剂、反应温度和底物浓度，获得最佳的反应条件为：正己烷作为溶剂，40℃下反应，底物浓度为100 mmol/L。在最佳的条件下，以乙酸乙烯酯作为酰基供体进行动力学拆分反应，经过后期的分离纯化，成功制备得到了e.e.值接近100%的(R)-3,5-双三氟甲基苯乙醇。%(R,S)-3,5-bis(trifluoromethyl)phenyl ethanol was prepared by reduction of 3,5-bis (trifluoromethyl) acetophenone with the NaBH4 as reducing agent. Two efficient and highly selective lipases,Novozym 435 and Rhizopus arrhizus,were screened with (R,S)-3,5-bis(trifluoromethyl)phenyl ethanol as substrate. Rhizopus arrhizus was selected as our experimental lipase and the factors which affected kinetic resolution were studied. When n-hexane was selected as solvent,optically pure (R)-3,5-bis (trifluoromethyl) phenyl ethyl acetate could be obtained after 30 min at 40 ℃. After post-processing,we obtained (R)-3,5-bis (trifluoromethyl) phenyl ethyl with the e.e. value of 100%.
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