基于HPLC-ABTS体系筛选细叶杜香抗氧化活性成分

摘要

细叶杜香(Ledum palustre L.var.Angustum E.Busch)是我国北方常用药用材料,有止咳祛痰、平喘、扩张血管等功效.本文利用HPLC-ABTS+在线筛选体系对细叶杜香茎部的抗氧化活性成分进行了筛选和分析检测,实验结果表明:细叶杜香茎部甲醇提取物中含有大量抗氧化活性成分,其中经二氯甲烷萃取后纯化的一个组分通过核磁共振波普分析鉴定为秦皮素,该研究为细叶杜香中更多抗氧化活性物质的筛选提供了基础性实验依据.%The plant Ledum palustre L var. Angustum E. Busch, a well known edible medicinal herb in North China, has been used to relieve a cough,relieved asthma and blood vessel dilatation. In this research, four major antioxidant compounds were screening and detected from this plant stem using an on-line HPLC-ABTS* screening system. Plant stem have active antioxidant compounds, one of them was isolated by HPLC and identified by NMR. The study gives basic experimental data screening more antioxidant compounds.