HPLC 法同时测定白花蛇舌草中对香豆酸和熊果酸的含量

摘要

A method for simultaneously determination of p-coumaric acid and ursolic acid in hedyotis diffusa was established. An Eclipse SB–C18 column (250 mm×4.6 mm,5 µm) was adopted with a mobile phase of methanol–0.05%formic acid at the flow rate of 1.0 mL/min. The detection wavelength was set at 310 nm and 210 nm for p-coumaric acid, ursolic acid, respectively, and the column temperature was 30oC. The calibration curves were linear in the ranges of 0.001 79–0.028 64 mg/mL(r=0.999 8) for p-coumaric acid and 0.021 3–0.340 8 mg/mL(r=0.999 6) for ursolic acid. The average recoveries of p-coumaric acid and ursolic acid were 98.59% and 98.23%,respectively,and the detection limit of p-coumaric and ursolic acid was 0.81%,0.94%(n=6),respectively. The sample solution was steady within 24 h. The method is suitable for determination of p-coumaric acid and ursolic acid in hedyotis diffusa.%建立高效液相色谱法同时测定白花蛇舌草药材中对香豆酸和熊果酸含量的方法。采用 Eclipse SB–C18色谱柱(250 mm×4.6 mm，5µm)，以甲醇–0.05%甲酸梯度洗脱，流量1 mL／min，对香豆酸和熊果酸的检测波长分别为310 nm 和210 nm，柱温30℃。对香豆酸和熊果酸含量分别在0.00179～0.02864 mg／mL 和0.0213～0.3408 mg／mL范围内与色谱峰面积呈良好的线性关系，线性相关系数 r 分别为0.9998，0.9996。对香豆酸和熊果酸的平均加标回收率分别为98.59%，98.23%，测定结果的相对标准偏差分别为0.81%，0.94%(n=6)。样品溶液在24 h 内稳定。该方法适用于白花蛇舌草药材中对香豆酸和熊果酸的含量测定。