A method for determination of benzopyrene in vegetable oil by High Performance Liquid Chromatography was established. Using C18 reversed-phase column, with acetonitrile and H2O (88∶12,v/v) as mobile phase,flow rate was 1.0 ml/min, and excitation wavelength was 384nm and emission wavelength was 406 nm, and column temperature was 35℃, and injection volume was 10μl. Results: The detection limit was 0.5μg/kg, and the recovery rate arange from 95% to 103%, and RSD was≤ 2.7%.%采用C18反相色谱柱,在流动相为乙腈∶水(88∶12,v/v),流速为1.0 ml/min,激发波长384 nm,发射波长406 nm,柱温35℃,进样量10μl 的条件下,建立高效液相色谱法快速测定植物油中苯并芘残留量的方法。结果:本法检出限为0.5μg/kg,加标回收率95%~103%,RSD≤2.7%。
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