According to the exogenous insert sequences connected with the border sequences of soybean endogenous genes, we designed and screened specific loop-mediated isothermal amplification primers,and optimized the reaction system and the reaction conditions using a design software named Primer Explorer Version 3, then established the specific loop-mediated isothermal amplification method for the detection of the transgenic soybean line GTS 40-3-2 strains. The specificity,stability and sensitivity of this method were evaluated. The results indicated that the transgenic soybean GTS 40-3-2 could be distinguished from various soybean strains and their derived products specifically,stably and sensitively with LOD of 0. 5%. In addition,the detection results of 12 samples were 100% consistent to that of the real-time PCR method,with the zero false positive and negative rates,which indicated that this method was equivalent to the real-time PCR method.%根据外源插入序列与大豆内源基因边界序列,用设计软件Primer Explorer Version 3设计并筛选了转基因大豆GTS 40-3-2品系的环介导等温扩增引物,并进行了反应体系和反应条件的优化,建立了转基因大豆GTS 40-3-2品系特异性环介导等温扩增检测方法.对该方法进行了特异性、灵敏度、稳定性评价,结果显示:该方法能够特异、稳定、灵敏地检测大豆及其制品中的转基因大豆GTS 40-3-2成分,检测低限达到0.5％.此外,对12份实际样品的检测结果表明,该方法与实时荧光PCR方法的检测性能相当,结果吻合率为100％,假阳性率和假阴性率均为0.
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