建立了蒽酮比色法快速测定复合硅铝酸盐霉菌吸附剂中酵母多糖含量的方法。根据复合硅铝酸盐的组成特点，确定的微波消解条件为：先在碱性条件下，温度60℃、时间20 min；后在酸性条件下，温度80℃、时间5 min，温度100℃、时间5 min，温度120℃、时间90 min。蒽酮比色法的显色条件为：加入蒽酮试剂7 ml，沸水浴时间9 min，最大吸收波长为630 nm。结果表明：在甘露糖溶液质量浓度为0．04～0．24 mg／ml时，线性关系良好（R2＝0．9981），相对标准偏差为0．15％，加标回收率为102％。%The rapid method of anthrone colorimetry was built to determinate zymosan levels in the compound aluminum sili-cate mycotoxin adsorbent. Conditions of microwave digestion were identified below based on the characteristics of compound a-luminum silicate:first, 60℃for 20 min under alkaline conditions;and then, 80℃for 5 min, 100℃for 5 min, 120℃for 90 min under acidic conditions. Color conditions of anthrone colorimetry were determined as:7 ml of anthrone reagent addition, 9min for boiling, and 630nm length of the maximum absorption. Results show that the linear relationship is well within the range of mannose 0. 04~0. 24 mg/ml(R2=0. 997 1), 0. 15%of RSD, and the 102%of recovery rate.
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