利用SRAP分子标记技术，对青花菜与其近缘种进行遗传多样性分析。28对SRAP引物共产生302条谱带，其中多态性谱带203条，多态率为67.22%，表明种质间存在较高的多态性。相似系数分析表明，其变异范围为0.4615-0.9006，平均遗传相似系数为0.6936。‘绿地’和‘矮抗青’之间的亲缘关系最远，遗传相似系数为0.4615；‘Wzvcst-09-224’和‘Wzvcst-09-225’亲缘关系最近，遗传相似系数为0.9006。聚类分析可将16个材料分为两大类，第Ⅰ类包括芸薹属甘蓝种蔬菜，第Ⅱ类为芸薹属白菜种。揭示了青花菜及其近缘变种间具有部分相似的遗传基础，亲缘关系较近。结果表明，同一地域或来源的材料间具有较为相近的遗传背景，亲缘关系相对较近。研究结果有助于青花菜与其近缘种间种质资源分类和优异基因利用，加速青花菜新品种选育进程。%In this study, we analyzed genetic diversity and relationship between broccoli and its related species by SRAP molecular markers. Using 28 SRAP primer combinations, a total of 302 amplified fragments were detected and 203 were polymorphic, with polymorphism rate 67.22%, indicating high polymorphism among these germplasm. Similarity coefficient analysis showed that the variation ranged from 0.461 5 to 0.900 6, and the average genetic similarity coefficient was 0.693 6.‘Lü di’and‘Ai kang qing’had the farthest genetic relationship with genetic similarity coefficient 0.461 5. By contrast, the relationship was closest between‘Wzvcst-09-224’and‘Wzvcst-09-225’, with genetic similarity coefficient 0.900 6. Cluster analysis divided 16 germplasm into two major clusters. Class I contained Brassica oleracea, and class II only had one member Chinese no heading cabbage. The result of cluster indicated that the genetic basis had more similarity between broccoli and its related species, with a closer relationship, comparing with Brassica campestris ssp. chinensis Makino. The results also showed that the same geographic or origin could make the germplasm had relatively similar genetic background and closer genetic relationship. The research could be helpful to germplasm classification and excellent genes utilization for broccoli and its relative species, to speed up the breeding process.
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