旨在提高丁二酸发酵生产水平，通过初筛、复筛从土壤中分离得到了高产丁二酸菌株，发酵结束后利用高效液相色谱法检测丁二酸的产率为34.45%。对菌株进行紫外诱变选育，结果表明，在底物葡萄糖的浓度为100 g/L时，丁二酸的产率提高了46%，达到了50.30%，残糖低于10 g/L。经形态学特征、生理生化指标测定以及16S rDNA序列分析等，鉴定该菌株为放线杆菌。%It was to improve the production of succinic acid. After the primary and secondary screening, a strain with high-yield of succinic acid was isolated from the soil. The succinic acid production of 34.45% was determined by the high performance liquid chromatography (HPLC)after the fermentation. Later, the UV mutagenic breeding of strains has been performed, resulting in the succinic acid production of 50.30% which increased by 46% in the substrate of glucose concentration of 100 g/L with the residual sugar lower than 10 g/L. After the determinations of morphological characteristics, physiological and biochemical index and 16S rDNA sequence analysis, the strain was identified asactinobacillus.
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