METHOD FOR OBTAINING HIGHLY PRODUCTIVE STRAIN FOR PROTEIN AND PRODUCTION OF PROTEIN USING SAID HIGHLY PRODUCTIVE STRAIN

摘要

PURPOSE:To obtain a strain capable of highly producing a specific protein, by transfecting a DNA having a gene sequence coding a protein into a host higher eucaryotic cell having a specific gene sequence in the chromosomic DNA. CONSTITUTION:A DNA having a gene sequence I for coding a specific protein, gene sequence II for coding dihydrofolate reductase derived from wild-type cells and gene sequence III for coding a protein for sorting out a transformant is transfected into a host higher eucaryotic cell having a gene sequence for coding a methotrexate-sensitive dihydrofolate reductase in the chromosomic DNA. The resultant transformant strain is then cultivated in the presence of a competitive inhibitor having dihydrofolate reductase activity to amplify both the gene sequences I and II at the same time.