应用高通量测序技术研究柽柳、獐茅土壤真菌多样性

摘要

应用454高通量测序技术对柽柳和獐茅地土壤真菌种类及多样性进行研究，并对其进行真菌多样性指数和相似性分析，为黄河三角洲真菌多样性深入的研究和盐碱地的改良提供基础数据和理论依据。结果表明，4个土样共获得5门17纲37目48科51属。OTU 数和多样性指数表明：柽柳上层（C1：0-20 cm）土壤真菌多样性最丰富，其 OTU 数、Shannon 指数、Chao1指数分别是獐茅下层（Z2：20-40 cm）相应指数的1.7倍、1.2倍和2.4倍。距离热图分析表明，Z1和 Z2真菌群落间的相似性最大， Unifrac metric 值为0.2699；C1和 C2真菌群落间相似性最小，Unifrac metric 值为0.6902。柽柳地土壤真菌较獐茅地土壤真菌丰富。%The study,which was related to the variety and diversity of soil fungi in the field planting Tamarix chinensis and Angiospermae,and the corresponding analysis of fungal diversity index and similarity were conducted by applying 454 high-throughput sequencing technology. It is aimed at providing the basic datas and theoretical gist for the futher study into the diversity of fungi in Yellow River Delta and the improvement of saline-alkali soil. And the result indicated four soil samples were divided into 5 phyla,17 classes,37 orders,48 families and 51 genus. The OTU number and diversity index showed that the diversity of soil fungal diversity was the richest in Tamarix chinensis surface(C1 :0-20 cm),which was accordingly 1.7 times,1.2 times and 2.4 times of OTU number,Shannon index and Chao1 index in Angiospermae substratum(Z2 :20-40 cm). In addition,the analysis of the distance heat map indicated the fungal communities of Z1 and Z2 had the most similarity,whose Unifrac metric value was 0.269 9 and the fungal communities of C1 and C2 have the least similarity,whose value was 0.690 2. In a word,the soil fungi in the field planting Tamarix chinensis was richer than one in the field planting Angiospermae.