绿液预处理玉米秸秆产纤维素酶的研究

摘要

Abstract：The effect of green liquid pretreated corn stover on cellulase production of Trichoderma reesei was investigated. Inspecting the influence of pretreatment conditions on cellulase production of corn stover at different cooking temperature, total alkali charge and sulfidity by orthogonal experiment, we could conclude from range and variance analysis that the extent of impact to cellucase production of green liquor pretreated corn stover was in turn total alkali charge, cooking temperature and sultidity. The optimum filter paper activity （FPA） reached 2.6 IU/mL. FPA productions from corn stover oretreated bv cooking temperature140℃ and 170 ℃, total alkali charge 4 %, sulfidity 0, 20 %, 30 % and 40 %, were comprehensively compared and evaluated. It was found that the optimum conditions, i. e. , cooking temperature 140℃, total alkali charge 4 %, sulfidity 0, might ensure the value of FPA on and avoid the loss of materials. The results showed that green liquid pretreatment for corn stoverwas feasible for cellulase production of Trichoderma reesei. Taking corn stover pretreated by cooking temperature 140 ℃, total alkali charge 4% and sulfidity 0 as carbon sources whose concentration was 12 g/L, FPA and β-glucosidase activity（β-GA） were up to 2.5 IU/mL and 1.3 IU/mL respectively. The enzyme production period was 5 days.%研究了绿液预处理玉米秸秆对里氏木霉产纤维素酶的影响。通过正交试验考察3个预处理条件对玉米秸秆产纤维素酶的影响,从极差和方差分析可知,对绿液预处理玉米秸秆产纤维素酶的影响程度由大到小依次是总碱量、蒸煮温度、硫化度,最大滤纸酶活（FPA）达到2.6 IU/mL。比较在蒸煮温度140℃和170℃,总碱量4%,硫化度0、20%、30%与40%下所产的FPA,经综合评定,在最优条件下蒸煮温度140℃,总碱量4%和硫化度0时可以尽量避免原料损失的前提下,保证FPA的大小,结果表明,里氏木霉利用绿液预处理玉米秸秆来产纤维素酶是可行的。以蒸煮温度140℃,总碱量4%和硫化度0的条件下绿液预处理得到的玉米秸秆为碳源,碳源浓度为12 g/L,FPA和β-葡萄糖苷酶活（β-GA）分别达到2.5 IU/mL和1.3 IU/mL,产酶周期5 d。