[Objective] Three different pretreatment methods were tested to study the effect on hydrolysis of cellulose from corn stalk . [ Method ] The dilute - acid , dilute - alkali and sodium sulfite anhydrous pretreatment of corn stalk were carried out, followed by cellulose enzymatic hydrolysis. [Result] The results showed that the optimum condition for enzymatic hydrolysis is at temperature of 50％ , pH of 4.8, ratio of solid to liquid 1 ∶30,enzyme concentration of 2. 7 g/L, and the hydrolysis time of 24 h. Under the optimized condition, the rate of enzymatic hydrolysis on com stalk pretreatment using sodium sulfite anhydrous was 39 .07 ％ , which is four multiple higher than that of corn stalk hydrolysis without pretreatment. [ Conclusion] Pretreatment destroyed the fibrous structure of com stalk significantly. Relative good rate of enzymatic hydrolysis on com stalk can be achieved by sodium sulfite anhydrous pretreatment .%[目的]比较使用不同预处理方法对媒介纤维素水解率的影响.[方法]用稀酸法、稀碱法、亚钠法对玉米秸秆进行预处理,再用纤维素酶对玉米秸秆中纤维素进行水解.[结果]在50℃、pH为4.8、固液比为1:30、酶浓度为2.7g/L、反应时间为24h的条件下,可获得较理想的酶解率.经亚钠预处理后的玉米秸秆,纤维素含量上升最多,酶解率最高,亚钠预处理后的酶解率达到39.07%,是未经处理的秸秆酶解率(9.8%)的4倍.[结论]预处理破坏了玉米秸秆的纤维素结构.采用亚钠法-酶法结合工艺处理玉米秸秆进行纤维素酶解可显著提高酶解率.
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