高效液相色谱法分析青荚叶中豆甾醇的含量

摘要

采用反相高效液相色谱法（ RP-HPLC ）测定青荚叶中豆甾醇的含量，色谱柱为 Thermo C18柱（250 mm ×4.6 mm，5μm），流动相为甲醇：水（97：3），流速1.0 mL/min，进样量20μL，检测波长210 nm。结果表明，豆甾醇进样量在0.216~2.16μg，其峰面积与浓度呈良好的线性关系（ R2=0.9995），回收率为97.79%。方法专属性强，准确可靠，可用于青荚叶中豆甾醇的含量测定。%Using reversed-phase high performance liquid chromatography( RP-HPLC )to determine the content of stigmasterol in Helwingia,the chromatographic column was Thermo C18 column( 250 mm × 4. 6 mm,5μm),the mobile phase of methanol:water(97:3),flow rate of 1. 0 mL/min,the injection vol-ume was 20 μL,the detection wavelength was 210 nm. Results showed that stigmasterol sample volume in 0. 216~2. 16μg range,there was a good linear relationship between the peak area and concentration(R2=0. 999 5),the recovery rate was 97. 79%. This method is specific,accurate and reliable,and can be used for content determination of stigmasterol in Helwingia.