建立测定青荚叶中木犀草素、肉桂酸含量的HPLC测定方法。采用反相高效液相色谱法( RP-HPLC),色谱柱为Thermo C18柱(250 mm ×4.6 mm,5μm),测定木犀草素：流动相为甲醇∶0.2%磷酸水溶液(55∶45),流速1.0 mL/min,进样量20μL,检测波长350 nm；测定肉桂酸：流动相为甲醇∶1.67%冰醋酸水溶液(50∶50),流速1.0 mL/min,进样量20μL,检测波长270 nm。结果表明,木犀草素进样量在3.4~54.4μg范围内,其峰面积与浓度呈良好的线性关系(R2=0.9999),样品回收率为99.67%,肉桂酸进样量在0.24~2.4μg范围内,其峰面积与浓度呈良好的线性关系(R2=0.9994),样品回收率为93.64%。方法专属性强,准确可靠,可用于青荚叶中木犀草素、肉桂酸的含量测定。%To establish a HPLC method for content determination of luteolin in Helwingia and cinnamic acid. Using reversed-phase high performance liquid chromatography ( RP-HPLC ) , the chromatographic column was Thermo C18 column (250 mm × 4. 6 mm,5 μm),mobile phase of methanol determination of luteolin∶0. 2% phosphoric acid solution (55∶45),the flow rate was 1. 0 mL/min,the injection volume was 20 μL, the detection wavelength was 350 nm;determination of cinnamic acid∶the mobile phase of methanol∶1. 67% glacial acetic acid aqueous solution (50∶50),the flow rate was 1. 0 mL/min,the injec-tion volume was 20 μL,the detection wavelength was 270 nm. Results showed that luteolin in the range of 3. 4~54. 4 μg, there was a good linear relationship between the peak area and concentration ( R2 =0. 999 9),sample recovery rate was 99. 67%,cinnamic acid was in the range of 0. 24~2. 4μg,there was a good linear relationship between the peak area and concentration (R2 =0. 999 4),sample recovery rate was 93. 64%. This method is specific,accurate and reliable,and can be used for content determination of luteolin in Helwingia and cinnamic acid.
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