一株枯草芽胞杆菌产抑菌物质发酵条件及代谢产物稳定性研究

摘要

枯草芽胞杆菌L7是从牛粪中选出的,它的代谢产物中的抑菌物质对多种病原菌具有强烈的抑制作用。通过对其抑菌物质产生的发酵条件研究认为,最佳发酵条件为pH值7.0、接种量4%、30℃恒温振荡培养箱培养,发酵时间约60h。其代谢产物中的抑菌物质在80～121℃下30min稳定,pH值在6.0～10.0范围内稳定,光照2～6h后抑菌活性下降。结论：菌株L7的发酵产物具有较强稳定性,可置避光、pH值中性、常温环境中短期保存,经提高活性、纯化等处理后可能作为新型天然防腐剂应用于食品领域,或应用于抗感染微生态制剂的生产。%Bacillus subtilis L7 that was isolated from cow dung and the antibacterial substance in its metabolites had strong inhibition on multi-pathogenic bacteria. The studies on the fermentation conditions for producing the antibacterial substance showed that the optimal fermentation conditions were as follows：pH of 7.0, culturing with 4% inoculum amount under constant-temperature oscillation incubator for 60 h at 30 ℃. The antibacterial substance in its metabolites was stable at 80~ 121 ℃ for 30 min under pH of 6.0~10.0. The antibacterial activity decreased under illumination conditions after 2~6 h. In conclusion, the fermented products of strain L7 had stronger stability, so it could be preserved under the environment of lucifuge,neutral pH and room temperature for a short term.And it might be used as a new kind of natural antiseptic in food industry or applied in the production of anti-infection micro -ecological preparation after treatments of increasing the activity and purifying.